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1

A general protocol for the generation of Nanobodies for structural biology

Pardon, Els, Laeremans, Toon, Triest, Sarah, Rasmussen, Søren G F, Wohlkönig, Alexandre, Ruf, Armin, Muyldermans, Serge, Hol, Wim G J, Kobilka, Brian K, Steyaert, Jan

Journal:

Nature Protocols

Year:

2014

Language:

english

File:

PDF, 1.22 MB

english, 2014

2

Functional Immobilization of a Ligand-Activated G-Protein-Coupled Receptor

Lars Neumann,, Thorsten Wohland,, Rebecca J. Whelan, Richard N. Zare, Brian K. Kobilka

Journal:

Year:

2002

Language:

english

File:

PDF, 185 KB

english, 2002

3

The Molecular Basis of G Protein–Coupled Receptor Activation

Weis, William I., Kobilka, Brian K.

Journal:

Annual Review of Biochemistry

Year:

2018

Language:

english

File:

PDF, 5.45 MB

english, 2018

4

Skeletal muscle hypertrophy and anti-atrophy effects of clenbuterol are mediated by the β2-adrenergic receptor

Richard T. Hinkle, Karen M.B. Hodge, David B. Cody, Russell J. Sheldon, Brian K. Kobilka, Robert J. Isfort

Journal:

Year:

2002

Language:

english

File:

PDF, 238 KB

english, 2002

5

A genetically engineered cell-based biosensor for functional classification of agents

Alexander M Aravanis, B.Derek DeBusschere, Andrzej J Chruscinski, Kristin H Gilchrist, Brian K Kobilka, Gregory T.A Kovacs

Journal:

Biosensors and Bioelectronics

Year:

2001

Language:

english

File:

PDF, 206 KB

english, 2001

6

Crystal structure of the µ-opioid receptor bound to a morphinan antagonist

Manglik, Aashish, Kruse, Andrew C., Kobilka, Tong Sun, Thian, Foon Sun, Mathiesen, Jesper M., Sunahara, Roger K., Pardo, Leonardo, Weis, William I., Kobilka, Brian K., Granier, Sébastien

Journal:

Year:

2012

Language:

english

File:

PDF, 1.78 MB

english, 2012

7

Nanobodies to Study G Protein–Coupled Receptor Structure and Function

Manglik, Aashish, Kobilka, Brian K., Steyaert, Jan

Journal:

Annual Review of Pharmacology

Year:

2017

Language:

english

File:

PDF, 4.12 MB

english, 2017

8

Structures of the M1 and M2 muscarinic acetylcholine receptor/G-protein complexes

Maeda, Shoji, Qu, Qianhui, Robertson, Michael J., Skiniotis, Georgios, Kobilka, Brian K.

Journal:

Year:

2019

Language:

english

File:

PDF, 1.82 MB

english, 2019

9

Cloning of the gene and cDNA for mammalian β-adrenergic receptor and homology with rhodopsin

Dixon, Richard A. F., Kobilka, Brian K., Strader, David J., Benovic, Jeffrey L., Dohlman, Henrik G., Frielle, Thomas, Bolanowski, Mark A., Bennett, Carl D., Rands, Elaine, Diehl, Ronald E.

Journal:

Year:

1986

File:

PDF, 568 KB

1986

10

The genomic clone G-21 which resembles a β-adrenergic receptor sequence encodes the 5-HT1A receptor

Fargin, Annick, Raymond, John R., Lohse, Martin J., Kobilka, Brian K., Caron, Marc G., Lefkowitz, Robert J.

Journal:

Year:

1988

File:

PDF, 449 KB

1988

11

10.1038/46040

Kobilka, Brian K., Hein, Lutz, Altman, John D.

Journal:

Year:

1999

Language:

english

File:

PDF, 196 KB

english, 1999

12

Crystal structure of the human β2 adrenergic G-protein-coupled receptor

Rasmussen, Søren G. F., Choi, Hee-Jung, Rosenbaum, Daniel M., Kobilka, Tong Sun, Thian, Foon Sun, Edwards, Patricia C., Burghammer, Manfred, Ratnala, Venkata R. P., Sanishvili, Ruslan, Fischetti, Robe

Journal:

Year:

2007

Language:

english

File:

PDF, 1.07 MB

english, 2007

13

The structure and function of G-protein-coupled receptors

Rosenbaum, Daniel M., Rasmussen, Søren G. F., Kobilka, Brian K.

Journal:

Year:

2009

Language:

english

File:

PDF, 2.51 MB

english, 2009

14

Crystal structure of the β2 adrenergic receptor–Gs protein complex

Rasmussen, Søren G. F., DeVree, Brian T., Zou, Yaozhong, Kruse, Andrew C., Chung, Ka Young, Kobilka, Tong Sun, Thian, Foon Sun, Chae, Pil Seok, Pardon, Els, Calinski, Diane

Journal:

Year:

2011

Language:

english

File:

PDF, 2.55 MB

english, 2011

15

Functional Selectivity and Classical Concepts of Quantitative Pharmacology

Urban, J. D., Clarke, W. P., von Zastrow, M., Nichols, D. E., Kobilka, B., Weinstein, H., Javitch, J. A., Roth, B. L., Christopoulos, A., Sexton, P. M.

Journal:

Journal of Pharmacology and Experimental Therapeutics

Year:

2006

Language:

english

File:

PDF, 633 KB

english, 2006

16

A medium for the maintenance of Chironomus tentans salivary glands in vitro

Conrad E. Firling, Brian K. Kobilka

Journal:

Journal of Insect Physiology

Year:

1979

Language:

english

File:

PDF, 3.09 MB

english, 1979

17

PDZ-domain arrays for identifying components of GPCR signaling complexes

Peter Day, Brian Kobilka

Journal:

Trends in Pharmacological Sciences

Year:

2006

Language:

english

File:

PDF, 87 KB

english, 2006

18

The Dynamic Process of β2-Adrenergic Receptor Activation

Nygaard, Rie, Zou, Yaozhong, Dror, Ron O., Mildorf, Thomas J., Arlow, Daniel H., Manglik, Aashish, Pan, Albert C., Liu, Corey W., Fung, Juan José, Bokoch, Michael P., Thian, Foon Sun, Kobilka, Tong Su

Journal:

Year:

2013

Language:

english

File:

PDF, 2.37 MB

english, 2013

19

INSIGHTS FROM IN VIVO MODIFICATION OF ADRENERGIC RECEPTOR GENE EXPRESSION

Rohrer, Daniel K., Kobilka, Brian K.

Journal:

Annual Review of Pharmacology

Year:

1998

Language:

english

File:

PDF, 178 KB

english, 1998

20

Adrenergic Receptors as Models for G Protein-Coupled Receptors

Journal:

Annual Review of Neuroscience

Year:

1992

Language:

english

File:

PDF, 1.01 MB

english, 1992

21

Muscarinic Receptors as Model Targets and Antitargets for Structure-Based Ligand Discovery

Kruse, A. C., Weiss, D. R., Rossi, M., Hu, J., Hu, K., Eitel, K., Gmeiner, P., Wess, J., Kobilka, B. K., Shoichet, B. K.

Journal:

Molecular Pharmacology

Year:

2013

Language:

english

File:

PDF, 3.28 MB

english, 2013

22

Structural Insights into the Dynamic Process of β2-Adrenergic Receptor Signaling

Manglik, Aashish, Kim, Tae Hun, Masureel, Matthieu, Altenbach, Christian, Yang, Zhongyu, Hilger, Daniel, Lerch, Michael T., Kobilka, Tong Sun, Thian, Foon Sun, Hubbell, Wayne L., Prosser, R. Scott, Ko

Journal:

Year:

2015

Language:

english

File:

PDF, 1.88 MB

english, 2015

23

Structural Insights into the Dynamic Process of β2-Adrenergic Receptor Signaling

Manglik, Aashish, Kim, Tae Hun, Masureel, Matthieu, Altenbach, Christian, Yang, Zhongyu, Hilger, Daniel, Lerch, Michael T., Kobilka, Tong Sun, Thian, Foon Sun, Hubbell, Wayne L., Prosser, R. Scott, Ko

Journal:

Year:

2015

Language:

english

File:

PDF, 65 KB

english, 2015

24

Cryo-EM structure of the activated GLP-1 receptor in complex with a G protein

Zhang, Yan, Sun, Bingfa, Feng, Dan, Hu, Hongli, Chu, Matthew, Qu, Qianhui, Tarrasch, Jeffrey T., Li, Shane, Sun Kobilka, Tong, Kobilka, Brian K., Skiniotis, Georgios

Journal:

Year:

2017

Language:

english

File:

PDF, 17.51 MB

english, 2017

25

Diverse GPCRs exhibit conserved water networks for stabilization and activation

Venkatakrishnan, A. J., Ma, Anthony K., Fonseca, Rasmus, Latorraca, Naomi R., Kelly, Brendan, Betz, Robin M., Asawa, Chaitanya, Kobilka, Brian K., Dror, Ron O.

Journal:

Proceedings of the National Academy of Sciences

Year:

2019

Language:

english

File:

PDF, 1.54 MB

english, 2019

26

Pendant-bearing glucose-neopentyl glycol (P-GNG) amphiphiles for membrane protein manipulation: Importance of detergent pendant chain for protein stabilization

Bae, Hyoung Eun, Cecchetti, Cristina, Du, Yang, Katsube, Satoshi, Mortensen, Jonas S., Huang, Weijiao, Rehan, Shahid, Lee, Ho Jin, Loland, Claus J., Guan, Lan, Kobilka, Brian K., Byrne, Bernadette, Ch

Journal:

Acta Biomaterialia

Year:

2020

File:

PDF, 2.50 MB

2020

27

Structural basis for GLP-1 receptor activation by LY3502970, an orally active nonpeptide agonist

Kawai, Takahiro, Sun, Bingfa, Yoshino, Hitoshi, Feng, Dan, Suzuki, Yoshiyuki, Fukazawa, Masanori, Nagao, Shunsuke, Wainscott, David B., Showalter, Aaron D., Droz, Brian A., Kobilka, Tong Sun, Coghlan,

Journal:

Proceedings of the National Academy of Sciences

Year:

2020

File:

PDF, 2.15 MB

2020

28

A device for separated and reversible co-culture of cardiomyocytes

Michael Q. Chen, R. Hollis Whittington, Peter W. Day, Brian K. Kobilka, Laurent Giovangrandi, Gregory T. A. Kovacs

Journal:

Biotechnology Progress

Year:

2010

Language:

english

File:

PDF, 749 KB

english, 2010

29

Amino and Carboxyl Terminal Modifications to Facilitate the Production and Purification of a G Protein-Coupled Receptor

Journal:

Analytical Biochemistry

Year:

1995

File:

PDF, 308 KB

1995

30

Site-Specific Fluorescence Labeling of the β2Adrenergic Receptor Amino Terminus

Anthony L. Parola, Sansan Lin, Brian K. Kobilka

Journal:

Analytical Biochemistry

Year:

1997

Language:

english

File:

PDF, 276 KB

english, 1997

31

Using synthetic lipids to stabilize purified β2 adrenoceptor in detergent micelles

Zhiping Yao, Brian Kobilka

Journal:

Analytical Biochemistry

Year:

2005

Language:

english

File:

PDF, 150 KB

english, 2005

32

Agonist-induced conformational changes in the β2 adrenergic receptor

Journal:

Chemical Biology & Drug Design

Year:

2002

Language:

english

File:

PDF, 167 KB

english, 2002

33

Linkage mapping of α-2 adrenergic receptor genes to mouse Chromosomes 2 and 5

Richard E. Link, Brian K. Kobilka, Gregory S. Barsh

Journal:

Mammalian Genome

Year:

1993

Language:

english

File:

PDF, 788 KB

english, 1993

34

Regulation of G-Protein Coupled Receptor Traffic by an Evolutionary Conserved Hydrophobic Signal

Tim Angelotti, David Daunt, Olga G. Shcherbakova, Brian Kobilka, Carl M. Hurt

Journal:

Year:

2010

Language:

english

File:

PDF, 1.46 MB

english, 2010

35

Gene Substitution/Knockout to Delineate the Role of α2-Adrenoceptor Subtypes in Mediating Central Effects of Catecholamines and Imidazolines

L. HEIN, L. E. LIMBIRD, R. M. EGLEN, B. K. KOBILKA

Journal:

Annals of the New York Academy of Sciences

Year:

1999

File:

PDF, 1.28 MB

1999

36

An intronless gene encoding a potential member of the family of receptors coupled to guanine nucleotide regulatory proteins

Kobilka, Brian K., Frielle, Thomas, Collins, Sheila, Yang-Feng, Theresa, Kobilka, Tong Sun, Francke, Uta, Lefkowitz, Robert J., Caron, Marc G.

Journal:

Year:

1987

File:

PDF, 1.18 MB

1987

37

Removal of phosphorylation sites from the β2-adrenergic receptor delays onset of agonist-promoted desensitization

Bouvier, Michel, Hausdorff, William P., Blasi, Antonio De, O'Dowd, Brian F., Kobilka, Brian K., Caron, Marc G., Lefkowitz, Robert J.

Journal:

Year:

1988

File:

PDF, 553 KB

1988

38

Behavioural and cardiovascular effects of disrupting the angiotensin II type-2 receptor gene in mice

Hein, Lutz, Barsh, Gregory S., Pratt, Richard E., Dzau, Victor J., Kobilka, Brian K.

Journal:

Year:

1995

File:

PDF, 1.21 MB

1995

39

Ligand-regulated oligomerization of β2-adrenoceptors in a model lipid bilayer

Fung, Juan José, Deupi, Xavier, Pardo, Leonardo, Yao, Xiao Jie, Velez-Ruiz, Gisselle A, DeVree, Brian T, Sunahara, Roger K, Kobilka, Brian K

Journal:

The EMBO Journal

Year:

2009

Language:

english

File:

PDF, 1.30 MB

english, 2009

40

Ligand-specific regulation of the extracellular surface of a G-protein-coupled receptor

Bokoch, Michael P., Zou, Yaozhong, Rasmussen, Søren G. F., Liu, Corey W., Nygaard, Rie, Rosenbaum, Daniel M., Fung, Juan José, Choi, Hee-Jung, Thian, Foon Sun, Kobilka, Tong Sun

Journal:

Year:

2010

Language:

english

File:

PDF, 3.47 MB

english, 2010

41

Structure of a nanobody-stabilized active state of the β2 adrenoceptor

Rasmussen, Søren G. F., Choi, Hee-Jung, Fung, Juan Jose, Pardon, Els, Casarosa, Paola, Chae, Pil Seok, DeVree, Brian T., Rosenbaum, Daniel M., Thian, Foon Sun, Kobilka, Tong Sun

Journal:

Year:

2011

Language:

english

File:

PDF, 1.03 MB

english, 2011

42

Coupling ligand structure to specific conformational switches in the β2-adrenoceptor

Yao, Xiaojie, Parnot, Charles, Deupi, Xavier, Ratnala, Venkata R P, Swaminath, Gayathri, Farrens, David, Kobilka, Brian

Journal:

Nature Chemical Biology

Year:

2006

Language:

english

File:

PDF, 324 KB

english, 2006

43

A monoclonal antibody for G protein–coupled receptor crystallography

Day, Peter W, Rasmussen, Søren G F, Parnot, Charles, Fung, Juan José, Masood, Asna, Kobilka, Tong Sun, Yao, Xiao-Jie, Choi, Hee-Jung, Weis, William I, Rohrer, Daniel K

Journal:

Year:

2007

Language:

english

File:

PDF, 162 KB

english, 2007

44

Toward understanding GPCR dimers

Parnot, Charles, Kobilka, Brian

Journal:

Nature Structural and Molecular Biology

Year:

2004

Language:

english

File:

PDF, 192 KB

english, 2004

45

Dosage-dependent switch from G protein-coupled to G protein-independent signaling by a GPCR

Sun, Yutong, Huang, Jianyun, Xiang, Yang, Bastepe, Murat, Jüppner, Harald, Kobilka, Brian K, Zhang, J Jillian, Huang, Xin-Yun

Journal:

The EMBO Journal

Year:

2006

Language:

english

File:

PDF, 627 KB

english, 2006

46

Genetic alteration of the α2-adrenoceptor subtype c in mice affects the development of behavioral despair and stress-induced increases in plasma corticosterone levels

Lähdesmäki, J, Rybnikova, E, Pelto-Huikko, M, Kobilka, B K, Scheinin, M, Sallinen, J, Haapalinna, A, MacDonald, E, Viitamaa, T

Journal:

Molecular Psychiatry

Year:

1999

Language:

english

File:

PDF, 167 KB

english, 1999

47

Conformational Dynamics of Single G Protein-Coupled Receptors in Solution

Bockenhauer, Samuel, Fürstenberg, Alexandre, Yao, Xiao Jie, Kobilka, Brian K., Moerner, W. E.

Journal:

Journal of Physical Chemistry B

Year:

2011

Language:

english

File:

PDF, 3.25 MB

english, 2011

48

Structure of the δ-opioid receptor bound to naltrindole

Granier, Sébastien, Manglik, Aashish, Kruse, Andrew C., Kobilka, Tong Sun, Thian, Foon Sun, Weis, William I., Kobilka, Brian K.

Journal:

Year:

2012

Language:

english

File:

PDF, 1.70 MB

english, 2012

49

Conformational changes in the G protein Gs induced by the β2 adrenergic receptor

Chung, Ka Young, Rasmussen, Søren G. F., Liu, Tong, Li, Sheng, DeVree, Brian T., Chae, Pil Seok, Calinski, Diane, Kobilka, Brian K., Woods, Virgil L., Sunahara, Roger K.

Journal:

Year:

2011

Language:

english

File:

PDF, 1.59 MB

english, 2011

50

Plasmon-Waveguide Resonance Studies of Ligand Binding to the Human β2-Adrenergic Receptor†

Devanathan, Savitha, Yao, Zhiping, Salamon, Zdzislaw, Kobilka, Brian, Tollin, Gordon

Journal:

Year:

2004

Language:

english

File:

PDF, 169 KB

english, 2004

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